Extended Data Fig. 5: Validation of predicted inhibitors of mycolic acid biosynthesis.
From: Large-scale chemical–genetics yields new M. tuberculosis inhibitor classes
Actual performance of predicted inhibitors of mycolic acid biosynthesis in an in vitro assay of inhibition of incorporation of14C-acetic acid into mycolic acid. The ratio of imaged pixel intensities for fatty acid methyl ester (FAME) and mycolic acid methyl ester (MAME) bands was indicative of inhibition activity, as shown by the isoniazid and ethionamide controls. Open circles show the independent biological replicates (n = 5 for vehicle control, n = 4 for BRD-1728 and BRD-7564, n = 3 for BRD-4384 and BRD-9942, n = 1 for ethionamide control, and n = 2 for all other conditions); filled circles indicate the mean pixel intensity ratio; error bars denote the 95% confidence interval of the mean (statistical significance determined by P < 0.05, two-sided Wald test).
