Extended Data Fig. 6: PCR for rat Mbp from myelin preparation.
From: Neuronal vulnerability and multilineage diversity in multiple sclerosis
a, Representative Coomassie stain of brain homogenate (Hom.) and purified myelin (P.M.) from adult rat brain (left). Western blots for myelin basic protein (Mbp), Mog, synaptophysin (Syp) and neurofilament heavy molecular weight (NF-H) (centre). PCRs of myelin basic protein (Mbp) and synaptophysin (Syp) transcripts in brain homogenate and purified myelin fractions (right). b, Densitometric quantification of myelin and homogenates prepared from n = 4 independent rat hemispheres for Coomassie (total protein), western blot proteins and PCRs shown in a of purified myelin fractions normalized to their respective homogenates. Data are median ± s.e.m. of the four biological replicates. Similar results were obtained with brain homogenate and purified myelin fractions not used in this study. P values were calculated from Students’s two tailed t-test with Welch’s correction. P <0.05 was considered significant.
