Extended Data Fig. 1: Sample and disease contribution of cell types captured by snRNA-seq.
From: Neuronal vulnerability and multilineage diversity in multiple sclerosis
a, Representative images selected from nuclear suspensions (control, n = 9) after ultracentrifugation and before capturing by 10x Genomics confirming DAPI nuclear counterstaining with presence of smaller and larger DAPI+ nuclei. Note that larger nuclei are co-stained with anti-NeuN antibody confirming neuronal origin (white arrowheads). b, Coloured t-SNE plots showing numbers of genes (left) and UMIs (right) per captured nuclei from control and MS samples. c, Coloured t-SNE plot visualizing nuclei from different lesion stages based on classic pathological MS lesion staging. Acute, acute chronic-active; chronic, chronic inactive; ctrl, control. d, Coloured t-SNE plots visualizing nuclei from samples with different levels of upper- and deep-layer cortical demyelination as well as subcortical demyelination. e, Representative t-SNE plots with cell-type specific marker genes for OL progenitor cells, stromal cells including pericytes, endothelial cells and leukocytes. For t-SNE plots, data shown from 9 control and 12 MS samples and a total of 48,919 nuclei.
