Extended Data Fig. 7: ASC or caspase-1 deficiency rescues embryonic lethality of mice expressing CASP8(C362S).
From: Caspase-8 is the molecular switch for apoptosis, necroptosis and pyroptosis
a, Expected, observed and weaned numbers of mice per genotype obtained from the indicated crossings. b, Representative images of 8-week-old mice (top) and spleen, axial and inguinal and mesenteric lymph nodes (middle) as well as splenic sections stained with H&E (bottom) from Casp8C362S/WTMlkl−/−Asc−/− (n = 3), Casp8C362S/C362SMlkl−/−Asc−/− (n = 3), Casp8C362S/WTMlkl−/−Casp1−/− (n = 3) and Casp8C362S/C362SMlkl−/−Casp1−/− (n = 3) 8-week-old mice. Scale bars, 300 µm. c, Immunofluorescence confocal images of endothelial cells treated with HTNCre and stained with an anti-ASC antibody after 24 h (top) Scale bar, 20 µm. Measurement of IL-1β levels in supernatants of endothelial cells after 24-h HTNCre treatment (bottom; n = 3 biologically independent replicates). Dots and circles represent individual biological replicates. Data are mean ± s.e.m. One-way ANOVA followed by Sidak’s post-analysis. Results are representative of two individual experiments. d, Ponceau staining of ileal lysates from 5-week-old mice (n = 2) (Fig. 4d). Lanes, individual mice.
