Extended Data Fig. 10: Comparison of densely and poorly infiltrated kidney tumours by PDL-1 staining and by quantitative immunofluorescence.

a, Representative patients with densely infiltrated and poorly infiltrated kidney tumours whose disease has not progressed or has progressed, respectively. Whole-slide scans are shown for haematoxylin and eosin, anti-PD-L1, and immunofluorescence (CD8, MHC-II, DAPI) stains, with zoomed insets of immunofluorescence data. Yellow circles highlight the ___location of tumour tissue on the haematoxylin and eosin slide. Yellow boxes highlight the areas shown in the zoomed insets of immunofluorescence images. Immunofluorescence data are quantitatively analysed and mapped to show the density of MHC-II⁺ cells and the XY ___location of CD8⁺ T cells in the rightmost panel. Anti-PD-L1 scans are marked as ++ (positive-high), + (positive-low), or – (negative), as scored by board-certified pathologists. b, Patients in a are highlighted in red (highly infiltrated, non-progressors) and grey (poorly infiltrated, progressors) to show the percentage of CD8 T cell infiltration by flow cytometry. c, PD-L1 staining was scored by board-certified pathologists as positive-high, positive-low and negative. There is no significant difference between the percent CD8 T cell infiltration amongst these categories by ANOVA with Holm–Sidak correction. Median value shown. d, Progression free survival for patients with positive-high (PD-L1 high), positive-low (PD-L1 low), and negative (PD-L1 negative) kidney tumours. There was no significant difference in progression-free survival between the groups by Mantel–Cox log rank test (P = 0.6106) or by log rank test for trend (P = 0.3374).