Extended Data Fig. 7: Dietary vitamin D₃ does not alter the frequency of colonic RORγ⁺ T_reg cells.

a, b, Beginning at 3 weeks of age, three groups of mice were fed special diets for 4 weeks. SPF mice were fed either a nutrient-rich or a minimal diet, and GF mice were fed the nutrient-rich diet. The levels of 1,25-dihydroxyvitamin D₃ in serum (a) and the colon (b) of these mice were determined by ELISA. c, SPF mice were fed a nutrient-rich diet, or a rich diet deficient in vitamin D₃ (VitD3) or vitamin A (VitA) at birth. Colonic T_reg cells were analysed after 7 weeks. Frequencies of RORγ⁺Helios⁻ in the colonic FOXP3⁺CD4⁺TCRβ⁺ T_reg cell population are shown. d, SPF mice were fed a nutrient-rich diet at birth and were either maintained on that diet or switched to a rich diet deficient in vitamin D₃ or vitamin A at 3 weeks of age. Colonic T_reg cells were analysed after 4 weeks. Frequencies of RORγ⁺Helios⁻ in the colonic FOXP3⁺CD4⁺TCRβ⁺ T_reg cell population are shown. Data are representative of two independent experiments. n represents biologically independent animals. Data are mean ± s.e.m. ***P < 0.001, one-way ANOVA followed by the Bonferroni post hoc test.

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