Extended Data Fig. 2: Expression of guidance GPCRs in male and female cells, differential GPR174 expression by naive and germinal-centre B cells, and lack of influence on CCR7 expression.
From: A GPR174–CCL21 module imparts sexual dimorphism to humoral immunity
a, Relative mRNA expression of Cxcr5, S1pr2, CCR7 and Gpr183 in male or female dsRed-expressing MD4 B cells, normalized against that in cotransferred male GFP-expressing MD4 B cells (Extended Data Fig. 1), 90 h after immunization. Data are shown as pairs of three individual experiments. b, Relative Gpr174 mRNA levels in indicated splenic B cell subsets, normalized against Gapdh expression. The results are shown as scatter plots of three biological replicates, with lines indicating mean, and are representative of two independent experiments with similar results. c, Surface CCR7 levels on B cells that were retrovirally transduced with a control or GPR174-expressing vector. GFP+, infected cells after transduction; GFP−, uninfected cells after transduction; representative of three independent experiments. Two-tailed paired (a) or unpaired (b) Student’s t-tests were used to calculate P values, given at the top of each graph; ****P < 0.0001.
