Extended Data Fig. 6: Characteristics of commensal-immunized and unimmunized serum.
From: Microbiota-targeted maternal antibodies protect neonates from enteric infection
a, Western blot analysis of serum IgG from pups born to Pantoea-1-immunized SPF mice shows epitopes of ETEC 6, Pantoea 1 and Enterobacter, similar in size to those in serum from pups born to Pantoea-1-immunized germ-free mice. b, Serum IgG of pups born to Pantoea-immunized germ-free dams cross-reacts with different Enterobacteriaceae isolates from different facilities. 1, Harvard SGM Pantoea; 2, ETEC 6; 3, Harvard SGM Enterobacter; 4, Bacteroides fragilis NCTC9343; 5, Charles River B6 Proteus mirabilis; 6, Charles River B6 E. coli isolate 1; 7, Charles River B6 E. coli isolate 2; 8, Charles River CD1 E. coli isolate 1; 9, Charles River CD1 E. coli isolate 2; 10, Taconic B6 E. coli isolate 1; 11, Taconic B6 E. coli isolate 2; 12, Taconic B6 Proteus isolate; 13, Taconic B6 Enterobacter isolate; 14, C. rodentium. c, Pronase-treated bacterial lysates blotted with serum IgG of pups born to Pantoea-immunized germ-free dams. The concentrations of pronase are specified in the figure. a–c, Proteins were detected using a goat anti-mouse IgG antibody. For gel source data, see Supplementary Fig. 1. d, Mouse milk IgG and IgA concentrations. Data are shown in μg ml−1. e, Mouse milk IgG titre against microbiota. Each line represents an independent mouse. d, Data are mean ± s.e.m.
