Extended Data Fig. 7: Proliferation of UD-SCC-2 cells is dependent on NSD3.
From: Molecular basis of nucleosomal H3K36 methylation by NSD methyltransferases
a, Western blot analysis with indicated antibodies of whole-cell lysates from wild-type or NSD3-depleted UD-SCC-2 cells as indicated. All data were reproduced in three independent experiments. b, Cell proliferation rates of UD-SCC-2 cells expressing CRISPR–Cas9 and two independent NSD3 sgRNAs or a control sgRNA. Data are represented as mean ± s.d. from n = 3 biologically independent samples. **P < 0.01, n.s., not significant, two-tailed unpaired Student’s t-test. Cell lines were numbered as follows: (1) sgControl, (2) sgNSD3-1, (3) sgNSD3-2. P values between the cells are as follows: P (1 versus 2) = 0.0046, P (1 versus 3) = 0.0029, P (2 versus 3) = 0.3249. c, Western blot analysis of NSD3-depleted UD-SCC-2 cells complemented with structure-guided NSD3 derivatives. All data were reproduced in three independent experiments. d, Validation of NSD3 antibody specificity. Left, transient expression of either vector alone or Flag-tagged NSD3 in HEK 293T cells. Right, CRISPR–Cas9-mediated knockdown of either control or NSD3 in MDA-MB-231 cells. Whole-cell lysates were blotted with indicated antibodies. *, non-specific band. All data were reproduced in three independent experiments. For gel source data, see Supplementary Fig. 1.
