Extended Data Fig. 11: γδ T cells expand in the tissues of prodrug-treated, E. coli-infected humanized mice.
From: RETRACTED ARTICLE: IspH inhibitors kill Gram-negative bacteria and mobilize immune clearance
a, Hu-mice infected with E. coli (green) and treated with TPP or the prodrug C23.07–TPP are compared for expansion of Vδ2 TCR+ T cells (red) in multiple organs at day 5 post-infection. DAPI, blue. Scale bar, 100 μm (representative of samples tested from 5–6 Hu-mice). b, Vδ2 antibody validated for immunofluorescence staining of formalin-fixed human PBMCs that are γδ expanded (HMBPP + IL15) or γδ depleted. HepG2 and Vero cells serve as negative controls. c, Anti-E. coli antibody validated for immunofluorescence staining of formalin-fixed HepG2 cells co-infected with E. coli BL21 strain. HepG2 without E. coli serves as a negative control.
