Extended Data Fig. 4
From: Mechanism of adrenergic CaV1.2 stimulation revealed by proximity proteomics
Two-way hierarchical clustering of scaled data from Fig. 2. a, Dendrogram showing two-way hierarchical clustering of scaled TMT s/n data for streptavidin-purified proteins from α1C–APEX2 cardiomyocytes after stimulation with vehicle or isoproterenol. Shown are scaled relative TMT protein quantification data for 1,951 proteins from biological quintuplicate α1C–APEX2 mice. Clustering used Ward’s minimum-variance method. b, Dendrogram showing two-way hierarchical clustering of scaled relative quantification data for 1,936 proteins from biological triplicate β2B–APEX2 experiments. Heterogeneity between cardiomyocyte preparations from different mice is apparent. c, Dendrogram showing two-way hierarchical clustering of scaled relative quantification data for 2,610 proteins from whole-organ α1C–APEX without or with perfusion of isoproterenol. Prominent heterogeneity in relative protein quantification between hearts is apparent. The position of Rad is indicated by a red line. In this experiment, the individual hearts were not paired. d, Dendrogram showing two-way hierarchical clustering of scaled TMT s/n data from non-transgenic mouse cardiomyocytes stimulated with isoproterenol or with vehicle. Scaled data for 4,622 quantified proteins from a biological quadruplicate experiment are displayed. Pairing of samples is apparent.
