Extended Data Fig. 7: Sequence alignment of ALG6 homologues and orthologues.
From: Structure and mechanism of the ER-based glucosyltransferase ALG6
a, Alignment of amino acid sequences of yeast and human ALG6 and ALG8 proteins, generated with Clustal Omega57 (Uniprot identifiers: Q12001, Q9Y672, P40351 and Q9BVK2). Secondary structure elements of yeast ALG6 are depicted and labelled above the sequence. The dashed lines indicate regions that are disordered in the ALG6 structures. Cytosolic regions are labelled ‘cyto’ and ER-luminal regions are labelled ‘ER-lumen’. The red dot indicates the catalytic base Asp69, which is a structurally conserved aspartate residue that has been observed or proposed to be the catalytic base in all GT-C enzymes of known structures. The black dots indicate every tenth amino acid in the sequence of S. cerevisiae ALG6. b, Surface representation of yeast ALG6 (light brown), with residues identical to human ALG6 highlighted in green (left) and residues identical to yeast ALG8 highlighted in blue (right).
