Extended Data Fig. 8: Data processing and structure determination of the substrate-bound ALG6–6AG9-Fab complex in detergent solution.
From: Structure and mechanism of the ER-based glucosyltransferase ALG6
a, Overview of the EM data processing and structure determination pipeline using RELION 3.046. b, A representative cryo-EM micrograph. c, Representative 2D classes. d, Spatial distribution of particles in the final iteration of 3D refinement. e, Resolution estimation of the final map via FSC, as calculated in RELION 3.0. f, The final refined and B-factor-sharpened map, coloured by local resolution estimation, as calculated in ResMap52. g, The FSC between model and map, as calculated in PHENIX mtriage51.
