Extended Data Fig. 3: Cryo-EM analysis of the free RSC complex.
From: Structure of SWI/SNF chromatin remodeller RSC bound to a nucleosome
a, Representative cryo-EM micrograph of the free RSC complex shows homogeneously distributed individual particles. b, Two-dimensional class averages of the free RSC complex. c, Cryo-EM processing workflow for the reconstruction of the free RSC complex. Particle distribution after 3D classifications is indicated below the corresponding map. The final maps after focused 3D refinement and masks are depicted in colour. Views are generally rotated by 180° with respect to Fig. 1c, right. d, Angular distribution plot for all particles contributing to the final reconstruction of the free RSC complex. e, Two views of the combined RSC core map coloured according to the local resolution as implemented in RELION42. f, FSC plots of the maps used for model building of the RSC core complex.
