Fig. 1: Profiling histone modifications during mouse fetal development.

a, Experimental design. b, Three major axes of the data series: data types, tissues, and developmental stages (chr11: 98,318,134–98,336,928; mm10). Horizontal scale 0–30 for narrow marks (H3K4me3, H3K4me2, H3K27ac, H3K9ac), 0–10 for broad marks (H3K27me3, H3K4me1, H3K9me3, H3K36me3) and ATAC–seq. c, Number of TSS-distal (top, >1 kb) and TSS-proximal (bottom) ATAC–seq peaks for each tissue. d, k-means clustering of H3K27ac peaks (n = 333,097) across tissue-stages (k = 8). Cluster sizes, top to bottom: 20,497, 50,790, 31,043, 36,849, 38,670, 31,168, 36,822 and 87,258. e, Spearman’s correlations of peak strength between replicates from the same stage (that is, developmental stages separating data sets is 0), or from different stages separated by one to six intervening stages, as indicated. Number of points per comparison: 0 stages, 66; 1 stage, 108; 2 stages, 84; 3 stages, 60; 4 stages, 36; 5 stages, 20; 6 stages, 10. For all boxplots in this paper: horizontal line, median; box, interquartile range (IQR); whiskers, most extreme value within ±1.5 × IQR.