Extended Data Fig. 8: Subcellular localization of Hodor.
From: An intestinal zinc sensor regulates food intake and developmental growth
a, Quantification of the fraction of Hodor-positive punctae that co-express Rab 5, 7, 11 (all of which are endogenously tagged with YFP), Lysotracker or Lamp1 (endogenously expressed Lamp1–mCherry). b–d, Co-expression analysis reveals limited overlap between Hodor immunoreactivity and the early endosome marker Rab5 (b) or the recycling endosome marker Rab11 (d), whereas more pronounced overlap is apparent with late endosome and lysosome marker Rab7 (c). e, The majority of Lamp1-positive structures co-expressed Hodor on the apical side of interstitial cells (* denotes the intestinal lumen). Larvae were briefly starved (4 h) before dissection in order to visualize lysosomes as punctate structures. f, The endogenously expressed GFP-tagged Vha16-1 subunit of the V-ATPase complex is predominantly localized to the copper cell region (#) within the larval intestine. g, Expression of Vha16-1–GFP is apparent in both the copper cells and, to a lesser extent, the interstitial cells. See Supplementary Information for sample sizes and full genotypes. Scale bars, 10 μm (b–e); 200 μm (f); 30 μm (g). N, nucleus. *P < 0.05, **P < 0.01, ***P < 0.001. Box plots: line, median; box, 75th–25th percentiles; whiskers, minimum to maximum.
