Extended Data Fig. 2: Characterization of human segmentation clock period in in vitro PSM.
From: Recapitulating the human segmentation clock with pluripotent stem cells
a, HES7 reporter activity in a 2D culture (the oscillation assay condition) and 3D spreading spheroid (the synchronization assay condition). Raw, detrended (± 100 min window) and phase signals are shown. For spheroids, the signal was averaged over all area or ROIs indicated by the red line. 2D culture data are same as Fig. 1g and part of 3D-spheroid culture data are same as Fig. 1h. Data of three independent experiments are shown. Schematic depiction of reporter construct is shown on top. b, Human segmentation clock period quantification based on detrended and instantaneous phase signals. The period was calculated as the average peak-to-peak interval using the 1st to 5th peaks. The measure of centre is mean, n = 3. c, Instantaneous phase-based kymograph of travelling-wave-like HES7 reporter activity in spheroid spreading assay shown in Fig. 1h. Representative data of three independent experiments are shown.
