Extended Data Fig. 5: The HIV-1 RRE forms two stable alternative structures in CD4+ T cells.
From: Determination of RNA structural diversity and its role in HIV-1 RNA splicing
a, Schematic of DMS treatment in primary cells and isolated virions. b, DMS-MaPseq probing of the intracellular HIV-1NL4-3 RRE in CD4+ T cells was used as input for DREEM clustering. Two clusters passed the BIC test and were used as constraints on the folding using RNAstructure. Structural models are colour-coded by normalized DMS reactivity; bases not covered by the region of PCR are coloured in grey. Data used to construct models are representative data from n = 2 biologically independent experiments.
