Extended Data Fig. 5: Inhibitors of cellular receptors and kinases are not toxic to 1HAEo⁻ cells.

a, Violin plot of data from the single-cell RNA sequencing analysis of relative EGFR expression levels in the various structural cell types of adult human lungs. EGFR is expressed primarily in basal cells. Dots indicate expression values for individual cells and the shape of the graph is the overall distribution of the data for each cell type, n = 1,572 individuals. b, c, Cells were treated with the cellular inhibitors tested in Figs. 1e, 2a. Solid lines indicate the mean of n = 3 biological replicates and the dotted line indicates viability of control cells (100%). Data shown are representative of 2 independent repeats. d, The efficacy and specificity of pre-treatments of 1HAEo⁻ cells with MEK1/2 kinase (UO126), PI3K (LY294002) and p38 MAPK (SB203580, BIRB 796) was determined in an entry time course of RSV infection. Protein levels in cell lysates were measured by western blot. SOD-1 or β-actin were used as loading controls. Data shown are representative of 4 independent experiments.