Extended Data Fig. 8: HSC colony data.
From: Single-molecule imaging of transcription dynamics in somatic stem cells
a, Endpoint cells are the leaves on each pedigree. Note that edge lengths are not scaled on time between divisions, and all endpoint cells are 96 h from the start of the experiment. Cells are colour-coded according to the colour scheme used throughout the manuscript. Megakaryocytes are labelled in orange. Nodes (cells) observed upstream of the endpoint (that is, no transcriptional data are available) are coloured black. b, Histogram of number of progeny from a single HSC. c–e, Proliferation phenotypes of cells based on endpoint state identity (P1H, n = 137; LES, n = 1,571; G1/2H, n = 81; G2H, n = 166). Cell lifetimes in e are the time interval between cell birth (last division) and the next cell division or cell death. Violin plots are normalized to area, with the centre box-and-whisker plots showing the mean (line), standard deviation (box) and 95% confidence interval (whiskers). In e, single dots represent outliers in the 99th percentile.
