Extended Data Fig. 8: Lipidomics companion to the decrease in MFSD2A expression in the aged brain vasculature.
From: Physiological blood–brain transport is impaired with age by a shift in transcytosis
a, PC analysis plot of brain microvessel lipidomes from young (3 mo) and aged (20 mo) mice. There were 25 lipids significantly upregulated and 33 lipids downregulated with age (n = 4, each n is a pool of 4 mice (16 young and 16 aged total), FDR-corrected q < 0.05, Permutation-based FDR of two-sided t-test). b, Absolute abundance (concentrations) of measured microvessel lipid classes with age (n = 4, each n is a pool of 4 mice, Benjamini, Krieger and Yekutieli FDR-corrected two-sided t-test; mean ± s.e.m.). DG is not listed as only one DG species was detected. c, Mole % of MFSD2A-regulated phospholipids19 (PC, PE, PS and LPE) across and within phospholipid classes. Concentrations of individual lipid species were derived with spike-in standards and presented as a quantitative mole % of DHA fatty acid (FA) of all fatty acids in the given class68 or across PC, PE, PS and LPE classes (right, ‘Combined’) (n = 4, each n is a pool of 4 mice, two-sided t-test; mean ± s.e.m.). d, Hierarchical clustering of lipids (by concentration) of microvessels from young (3 mo) and aged (20 mo) mice (n = 4; each sample is a pool of 4 mice) normalized by Z-score. All lipids differentially abundant with an FDR-corrected q < 0.1 are shown (permutation-based FDR of two-sided t-test), and MFSD2A-regulated DHA-phospholipids are labelled.
