Extended Data Fig. 7: B cell development in Wapl mutant mice.
From: Wapl repression by Pax5 promotes V gene recombination by Igh loop extrusion
a, b, Wapl expression in ex vivo-sorted Wapl∆P1/∆P1 (blue), Wapl∆P1/+ (light blue) and Wapl+/+ (black) pro-B and pre-B cells (a) as well as in Wapl∆P2/∆P2 (blue) and Wapl+/+ (black) pro-B cells (b), as determined by RT–qPCR analysis relative to Tbp expression. The Wapl expression of each cell type is shown as mean value with s.e.m. relative to that of Wapl+/+ cells (set to 1). Each dot corresponds to one RT–qPCR experiment performed with sorted cells from one mouse. c, d, Flow cytometric analysis of bone marrow from Wapl∆P2/∆P2 (blue), Wapl∆P2/+ (light blue) and Wapl+/+ (black) mice (c). The frequencies of the indicated cell types are shown as mean values with s.e.m. (d). e, Flow cytometric analysis of bone marrow from Cd79acre/+Waplfl/fl and Cd79acre/+Wapl+/+ mice. f, g, Frequencies of total B, pro-B and pre-B cells are shown for Cd79acre/+Waplfl/fl, Cd79acre/+Waplfl/+ and control mice (f) as well as for Rag1cre/+Waplfl/fl, Rag1cre/+Waplfl/+ and control mice (g). The control mice in f were 3 × Cd79acre/+Wapl+/+ and 5 × Waplfl/+ mice, and the control mice in g were 2 × Rag1cre/+Wapl+/+, 2 × Waplfl/fl, 1 × Waplfl/+ and 1 × Wapl+/+. Statistical data (a, b, d, f, g) are shown as mean values with s.e.m. and were analysed by multiple t-tests (unpaired and two-tailed with Holm–Sidak correction). One of 5 (c) or 4 (e) experiments is shown. n, number of mice. Each dot corresponds to one mouse.
