Extended Data Fig. 8: Ubiquitination of E promotes virus–endosome membrane fusion.
From: Envelope protein ubiquitination drives entry and pathogenesis of Zika virus
These experiments were performed in JEG-3 cells. a–c, Wild-type ZIKV, E(K38R)- and E(K281R)-mutant ZIKV were labelled with DiOC18. After filtration, viruses were incubated at 4 °C with JEG-3 cells at MOI 2, and after 30 min were washed and collected for controls as adsorbed viruses. Additional samples were then incubated at 37 °C for 1 h, in the presence or absence of NH4Cl to block acidification (as control), washed, fixed and visualized with a confocal microscope (a). The same experiment was repeated for quantification by fluorescence-activated cell sorting (FACS). Mean fluorescence intensity (MFI) is shown in b; the percentage of cells infected is shown in main Fig. 3a; and representative histograms are shown in c. n = 3 technical replicates, mean, unpaired two-sided t-test, *P < 0.05. Representative of two independent experiments.
