Extended Data Fig. 2: Fourier shell correlation of cryo-EM maps, orientation distribution of particle image views, and local resolution of TRPA1 cryo-EM maps.
From: Irritant-evoked activation and calcium modulation of the TRPA1 receptor
a, Fourier shell correlation and 1D directional Fourier shell correlation plots. TRPA1 (PMAL) + A-96 class 2 denotes the structure derived from 3D classification of antagonist-treated samples in PMAL and repre\sents the open state channel without antagonist bound. b, Three-dimensional representations of the directional Fourier shell correlation. c, Fourier space covered, based on dFSC at 0.143. d, Orientation distribution of particle image refinement angles. e, The A-loop is lower resolution than surrounding map regions, indicating its dynamic nature. In the activated (TRPA1 + iodoacetamide) and open (TRPA1 + A967079 PMAL-C8 class 2) state conformations, the bottom of S6 is lower resolution than surrounding regions, indicating structural flexibility at the level of the lower gate. Scale bars, 5 Å.
