Extended Data Fig. 1: Bioengineering intestinal stem cell epithelia with a tubular, in-vivo-like architecture.
From: Homeostatic mini-intestines through scaffold-guided organoid morphogenesis
a, Photograph of the fully assembled microchip system. b, Schematic cross-sectional view of laser ablation using a nanosecond-pulsed laser. c, Hybrid collagen I/Matrigel scaffold in the central chamber before and after microchannel ablation. Scale bars, 200 μm. d, Fluorescence confocal images of a representative three-day old epithelial tube. Cells are labelled with DAPI (blue, nuclei) and E-cadherin (green). Images correspond to the maximal intensity projection of a z-stack of 100 μm. Scale bars, 50 μm. Data are representative of at least two independent experiments.
