Extended Data Fig. 1: Characterization of 4sT.
From: Conformation of sister chromatids in the replicated human genome
a, Synthetic hairpin-oligonucleotide used to probe 4sT conversion by OsO4. The theorized reaction educts and products are highlighted in red. b, High performance liquid chromatography (HPLC) trace at 260 nm of the oligos depicted in a before and after the conversion by OsO4/NH4Cl. The peak position of the oligo before conversion is indicated by a dashed line. c, Melting point analysis of a synthetic DNA hairpin containing 4sT or thymidine in the Watson–Crick base-paired stem (see Methods for details). d, Native mass spectrum of the oligonucleotide shown in a before and after OsO4/NH4Cl conversion measured in negative ion mode.
