Fig. 1: Cell composition of the adult human heart.
a, Transmural samples were obtained from left and right atrium, left and right ventricles, apex and interventricular septum from 14 individuals. Single nuclei (n = 14) and single cells (n = 7) were processed using Chromium 10x 3′DEG chemistry. b, Infographic shows donors (women, top; men, bottom), age, and contribution to cells and nuclei datasets (orange circle). Data are available in Supplementary Table 1. c, Uniform manifold approximation and projection (UMAP) embedding of 487,106 cells and nuclei delineate 11 cardiac cell types and marker genes. d, Distribution of cell populations, identified from nuclei within atria (left and right) and ventricles (left, right, apex and interventricular septum) after subclustering analysis. Colour code as in c. Data are available in Supplementary Table 2. Adip, adipocytes; Lym, lymphoid; Meso, mesothelial cells; Myel, myeloid; NC, neuronal cells; PC, pericytes. e, Multiplexed smFISH of cell type-specific transcripts in right ventricles (RV; left): TTN (green, cardiomyocytes) and CDH5 (red, EC) right atrium (RA; middle): NPPA (green, aCM) and DCN (red, FB) and left atrium (LA; right): MYH11 (green, SMCs) and KCNJ8 (red, pericytes). Nuclei are counterstained with DAPI (dark blue). Scale bars, 20 μm. For details on statistics and reproducibility, see Methods.
