Extended Data Fig. 9: Metabolite levels correlate with thermal stability of enzyme producing or using the metabolite.
a, b, Scatter plot of metabolite log2 fold-changes in mutant compared to wild-type strain (y axis) and protein abundance (a) or thermal stability (b) in each mutant for enzymes that directly interact with the metabolite (x axis) (n = 19 mutants, except for G6P/F6P–PhoA (n = 7), 2-oxoglutarate–SucA (n = 18), succinate–SdhD (n = 12), malate–FumA (n = 6), and malate–FumB (n = 12)). r depicts the Pearson correlation coefficient for each metabolite-enzyme pair. Black line represents the linear fit and grey shades the 95% confidence interval of the fit. c, Twenty strains used for targeted metabolomics analysis. d, Distribution of Pearson correlation coefficients for metabolite levels in each mutant and abundance or thermal stability of enzymes that directly interact with the metabolite (upstream and downstream of metabolite, as in a and b). Box plots are as in Fig. 2a. With all data represented on top of the box plots (nG6P/F6P = 6, nPEP = 5, nPyruvate = 8, n2-oxoglutarate = 4, nSuccinate = 6, nMalate = 9).
