Extended Data Fig. 8: YF17D-specific immune responses in hamsters, mice and macaques.

a, Correlation analysis of NAb titres determined by using YF17D (PRNT) and YF17D–mCherry (SNT) for a panel of 21 antisera (historical samples from previous vaccination studies in mice and hamsters). SNT₅₀ and PRNT₅₀ values were plotted to determine the correlation between both neutralization assays with a Pearson regression coefficient of 0.68 (P = 0.0006). b, c, NAb titres in hamsters (n = 12 from 2 independent experiments for all groups) (b) and Ifnar^−/− mice (YF-S1/2 (n = 11), YF-S0 (n = 11), YF-S1 (n = 8), sham (n = 9) and YF17D (n = 8) from 2–3 independent experiments) (c); sera collected at day 21 after vaccination in both experiments (two-dose vaccination schedule; Figs. 1, 2). d, Ifnar^−/− mice vaccinated according to a two-dose vaccination schedule (YF-S1/2 (n = 11), YF-S0 (n = 10), YF-S1 (n = 7), sham (n = 9) and YF17D (n = 8) from 3 independent experiments). Spot counts for IFNγ-secreting cells per 10⁶ splenocytes after stimulation with a YF17D NS4B peptide mixture. e, f, NAb titres after vaccination in macaques with YF-S0 (e) or placebo (f) (6 macaques per group from a single experiment); sera collected at indicated times after vaccination (two-dose vaccination schedule; Fig. 3). g, Ifnar^−/− mice vaccinated according to a single-dose vaccination schedule (YF-S0 (n = 8), sham (n = 5) and YF17D (n = 5) from 2 independent experiments). Spot counts for IFNγ-secreting cells per 10⁶ splenocytes after stimulation with a YF17D NS4B peptide mixture. Data are median ± IQR. Two-tailed uncorrected Kruskal–Wallis test was applied.

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