Extended Data Fig. 3: Attenuation of YF-S vaccine candidates.

a, Survival and weight curve of suckling BALB/c mice inoculated intracranially with 100 PFU of the different vaccine constructs. Mice were inoculated with YF-S1/2 (n = 8), YF-S0 (n = 8), YF-S1 (n = 10), sham (n = 10) or YF17D (n = 9). b, Representative images of BALB/c mice at seven days after intracranial inoculation with sham, or 10² PFU of either YF-S0 or YF17D. c, Survival and weight curve of AG129 mice (n = 8 for each group) after intraperitoneal inoculation with a dose range of YF-S0 (10², 10³ and 10⁴ PFU) (green) in comparison to YF17D (1, 10 and 10² PFU) (black and grey). d, Survival curve of wild-type (WT) and STAT2-knockout (STAT2^−/−) hamsters inoculated intraperitoneally with 10⁴ PFU of YF17D or YF-S0. Wild-type hamsters inoculated with YF17D (n = 6) and YF-S0 (n = 6); STAT2^−/− hamsters inoculated with YF17D (n = 14) and YF-S0 (n = 13). The number of surviving hamsters at study end point is indicated (a, c, d). e, f, Vaccine virus RNA (viraemia) in the serum (e) and weight evolution (f) of wild-type hamsters after intraperitoneal inoculation with 10⁴ PFU YF17D (n = 6) or YF-S0 (n = 6). The number of hamsters that showed viraemia on each day after inoculation is indicated below (e). g, Weight evolution of Ifnar^−/− mice after intraperitoneal inoculation with 400 PFU each at day 0 and 7 of YF-S0, YF17D and sham. Mice were inoculated with YF17D (n = 5), YF-S0 (n = 5) or sham (n = 5). Data in d are from two independent experiments, data in other panels are from a single experiment.

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