Extended Data Fig. 1: Immune-cell characteristics in livers of mice fed a CD-HFD.
From: Auto-aggressive CXCR6+ CD8 T cells cause liver immune pathology in NASH
a–e, Liver damage after 12 months of feeding a CD-HFD (which induces features of NASH) in Ja18−/− and Cd1d−/− mice, determined by sALT and NAS with representative images of H&E staining (mice fed a normal diet, n = 8; CD-HFD-fed, wild-type mice, n ≥ 7; CD-HFD-fed, Ja18−/− mice, n ≥ 7; CD-HFD-fed, Cd1d−/− mice: n ≥ 8). Scale bar, 100 μm. Two independent experiments. f, Body weight of mice over the course of six months. g, h, Numbers of hepatic lymphocytes (g) and hepatic CXCR6+ T cell populations (h) in mice fed a normal diet (n = 11) or CD-HFD (n = 13). Two independent experiments.i–k, Numbers and phenotype of hepatic CD44+ CXCR6+ and CXCR6− CD8 T cells in NASH mice (n ≥ 5). Two independent experiments. l, Frequencies of CXCR6+GzmB+ CD8 T cells in different organs in mice fed a normal diet (n = 4) and NASH mice (n = 4). m–o, Localization and quantification of hepatic CXCR6+PD1high CD8 T cells in mice fed a normal diet (n = 3) and NASH mice (n = 3), by confocal microscopy with representative images. Scale bar, 200 μm. CD3 (yellow), CD8 (purple), CXCR6 (cyan), PD1 (red) and collagen IV (white). Rectangles highlighted in m indicate representative zoomed areas shown in Fig. 1g. ns, not significant; nd, not determined. Exact P (a–d, f–l, n, o) and n (a–d, f, i–k) values are presented in Source Data. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Two-way ANOVA with Sidak’s (g, h, l) or with Tukey’s multiple comparison test (j, k), one-way ANOVA with Tukey’s (a–d) or with Dunnett’s multiple comparison test (f) and unpaired two-tailed t-test (i, n, o). In a–d, f–l, n, o, data are mean ± s.e.m., error is reported as s.d.
