Extended Data Fig. 2: Increased numbers of hepatic CXCR6+PD1high CD8 T cells in different mouse models of obesity-related NASH.
From: Auto-aggressive CXCR6+ CD8 T cells cause liver immune pathology in NASH
a, b, Confocal microscopy of hepatic PD1high CD8 T cells and IMARIS-based quantification with representative images (normal diet, n = 5; high-fat diet (HFD), n = 4; CD-HFD, n = 6). Scale bars, 500 μm (left), 100 μm (right). CD8 (red), PD1 (green), CK19 (blue), IBA1 (cyan), αSMA (purple) and DAPI (white). Rectangles highlighted in the left panel indicate represent zoomed areas in the right panel. Red arrows indicate PD1− CD8 T cells. Yellow arrows indicate PD1high CD8 T cells. One-way ANOVA with Dunnett’s multiple comparison test. c, d, Correlation of liver damage (NAS) with frequencies of hepatic PD1high CD8 T cells from mice fed a normal diet or CD-HFD. Coefficient of determination (R2) and statistical significance (P value) were determined using Pearson’s correlation. e, f, CXCR6 and PD1 expression levels in hepatic CD8 T cells in mice fed CD-HFD (e) or a high trans-fat Western diet (WD) (f) (n = 5) for 12 months compared to mice fed a normal diet (n = 5). h, i, Liver damage (sALT and NAS) in mice from f. j, k, CXCR6 and PD1 expression levels in hepatic CD8 T cells in ob/− and ob/ob mice after 4 months of chow feeding or mice fed a normal diet (ob/−, n = 6; ob/ob, n = 4). l, m, Liver damage (sALT and NAS) in mice from j. Exact P values (b, f–m) are presented in Source Data. *P < 0.05, **P < 0.01, ****P < 0.0001. Unpaired two-tailed t-test (f–m). In b, f–m, data are mean ± s.e.m., errors are shown as s.d.
