Extended Data Fig. 3: Notum is required for Apc-mutant cells to form intestinal tumours.
From: NOTUM from Apc-mutant cells biases clonal competition to initiate cancer
a, Survival plot for Lgr5CreER;Apcfl/fl;Notum+/+ (Notum+/+) and Lgr5CreER;Apcfl/fl;Notumfl/fl (Notumfl/fl) mice aged until clinical end point following induction with 0.15 mg tamoxifen. (n = 10 Notum+/+ mice, n = 10 Notumfl/fl mice). P = 0.12, log-rank test. b, Total small intestinal tumour burden (area) per mouse from mice in a (n = 10 Notum+/+ mice, n = 9 Notumfl/fl mice). c, Small intestinal tumour number per mouse from mice in a (n = 10 Notum+/+ mice, n = 10 Notumfl/fl mice). d, Representative H&E and Notum-ISH staining on serial sections from Notum+/+ and Notumfl/fl mice in a. The asterisks denote intestinal adenomas. The boxed areas are close-ups of adenomas stained for Notum. Note that adenomas grow out as Notum-positive lesions in Notumfl/fl mice, suggesting that retaining Notum confers a survival advantage during adenoma development. Scale bars, 200 μm. Data are mean ± s.e.m. In, b, c, Mann–Whitney U-test; P values are shown in the corresponding panels.
