Extended Data Fig. 7: Escapability and the relationships among antibody properties.
From: SARS-CoV-2 RBD antibodies that maximize breadth and resistance to escape
a, Additional spike-VSV viral escape selections, as in Fig. 3a, and an illustration of the authentic SARS-CoV-2 escape data for VIR-7832 (derived from S309) reported in Cathcart et al. 22. b, Correlation between the number of unique mutations selected across viral escape selection experiments and antibody escapability as tabulated in Fig. 1b, c, plus S2X25937. c, Projected epitope space from Fig. 4a annotated by antibody properties as in Fig. 4b–d. For each property, antibodies are coloured such that purple reflects the most desirable antibody (scale bar, right; N.D., not determined): narrowest functional epitope, tightest binding affinity (KD, log10 scale), lowest escapability. d, Pairwise scatter plots between all antibody properties discussed in the main text. Select scatter plots from this panel are shown in Figs. 4e–g. Details of each property described in Methods. All axes are oriented such that moving up on the y-axis and right on the x-axis corresponds to moving in the “preferred” direction for an antibody property (lower neutralization IC50, lower KD, higher breadth, narrower epitope size, lower escapability, lower total frequency of SARS-CoV-2 escape mutants among sequences on GISAID).
