Fig. 9: An integrated multimodal census and atlas of MOp cell types.
From: A multimodal cell census and atlas of the mammalian primary motor cortex
a, Mouse MOp consensus transcriptomic taxonomy at the top is used to anchor cell-type features in all the other modalities. Major cellular divisions, class and subclass labels are shown above major branches and cluster labels are shown below each leaf node. Using Patch-seq and connectivity studies, many transcriptomic neuron types or subclasses are annotated and correlated with known cortical neuron types. No Patch-seq data were collected for the ‘uncharacterized’ Vip types. Relative proportions of all cell types are calculated from the snRNA-seq 10x v3 B data (bar graph). b, Representative local dendritic and axonal morphologies of GABAergic and glutamatergic neuron types from Patch-seq data. c, UMAP representation of the mouse transcriptomic–epigenomic integrated molecular taxonomy (SCF version). d, Gradual transition of MERFISH IT clusters across cortical layers and depth. e, Percentage of LIGER, MERFISH and human cells assigned to mouse consensus transcriptomic cell types (Methods). Darker subclass colours indicate an exact match to the cluster/type, while lighter-coloured stacked bars indicate a match to taxonomic neighbours within the same subclass or, occasionally, a neighbouring subclass. Grey line, mean exact type match over neuronal types; black line, mean subclass match. f, Single-neuron full morphology reconstructions show distinct long-range axon projection patterns between glutamatergic subclasses and cell-to-cell variations within each subclass: L2/3 IT (17 cells), L4 IT (3 cells), L5 IT (5 cells), L5 ET MY-projecting (6 cells) and L5 ET non-MY-projecting (6 cells). Left, Allen mouse brain CCFv3 as an anatomical reference. CCK, cholecystokinin; FS, fast spiking; NGC, neurogliaform cell; PV, parvalbumin; SST, somatostatin; VIP, vasoactive intestinal peptide.
