Extended Data Fig. 9: Additional information about mutations in miR-26a.

a) Table depicting the name and sequence of the different EXOmotifs introduced in miR-26a-5p. Bold underlying text in the sequence indicates changed nucleotide/s in the guide strand of the miRNA. Nucleotides in the passenger strand were also modified accordingly to maintain miRNA structure. b) Predicted structure for the hairpin miRNA for each of the constructs shown in a. Red means high probability of pairing while blue indicates low probability calculated by RNAfold WebServer software. Arrows indicate the ___location of the mutated nucleotide/s. c) Normalized expression for miR-26a-5p wild-type in cells and sEV for each cell type. Average Ct from the whole miRNA profile was used for normalization for each sample. *P≤0.05 (Limma t-test). d) sEV enrichment calculated as the ratio of sEV expression divided by cellular expression for each of the constructs expressed in and secreted from SVEC endothelial cells. The dashed line separates preferential sEV enrichment (above line) versus preferential cellular enrichment (below line). Expression was normalized to the expression of miR-501-5p. *P≤0.05 (Kruskal-Wallis followed by Mann-Whitney U test), n=3-4.