Extended Data Fig. 4: Location of EXO and CELLmotifs and comparison between small miRNAseq (smRNAseq) and qPCR-based miRNA profiling.

a, b) Percentage of miRNAs showing indicated EXOmotifs (a) or CELLmotifs (b) either in the 5′ half (nucleotides 1-9, light yellow bars) or 3′ half (from nucleotide 10 to the 3′ end, orange bars) of the miRNA sequence. c) sEV and cell lysates from differentiated 3T3-L1 white adipocytes and AML12 hepatocytes were subjected to smRNAseq or qPCR-based profiling (n=4 for each cell type and compartment). Detected miRNAs by each method in each cell type correspond to the sum of black (non-selectively distributed miRNAs) and blue bars (selectively distributed miRNAs either in sEV-enriched or cell-enriched). The percentages in the blue bars refer to the ratio between the number of selectively distributed miRNA and the total number of detected miRNAs for each method and cell type. d) Venn diagrams indicating the number of miRNAs with a selective distribution in sEV or cells detected by smRNAseq (blue circles) and qPCR (green circles) in 3T3-L1 (above) or AML12 (below). The total number of miRNAs detected simultaneously by these two methods was 180. e) Table depicting the top EXOmotif found by HOMER software in sEV-enriched miRNAs from 3T3-L1 and AML12 detected by smRNAseq. Fold enrichment refers to the ratio between presence in the sEV-enriched miRNAs and presence in the rest of miRNAs (background).