Extended Data Fig. 7: Expression of viral RNA and protein for SARS-CoV-2 variants.
From: Evolution of enhanced innate immune evasion by SARS-CoV-2
a, Log2 ratio of Alpha to IC19 subgenomic RNA (sgRNA) abundance as determined from the RNA-seq dataset. b, Log2 ratio of Alpha to IC19 viral proteins. Peptide intensities are summed per viral protein (n = 3). c, Quantification of sgRNAs for M, S, Orf8, Orf7a, Orf3a, E and N* from the RNA-seq dataset. Counts are normalized to genomic RNA abundance at each time point and virus. d, Quantification of Orf3a (left) or S (right) sgRNA abundance via RT–qPCR. e, Summed peptides per viral protein for M, S, Nsp1, Orf7b, and Orf3b. f, Western blot quantification of Orf6 and N protein in infected cells at 24 hpi (n = 3). g, Pie chart depicting proportion of total sgRNA mapping to each viral sgRNA for IC19. h, Mean ± s.e.m. are shown. Comparison of percentages of total sgRNA mapping to each viral sgRNA across Alpha, VIC, and IC19. * (p < 0.05), ** (p < 0.01), *** (p < 0.001), **** (p < 0.0001). ns: non-significant, ND, not detected.
