Extended Data Fig. 2: The SARS-CoV-2 Alpha variant antagonizes innate immune activation more efficiently than early-lineage isolates.
From: Evolution of enhanced innate immune evasion by SARS-CoV-2
a, IFNβ gene expression (left) and protein secretion (right) from cells in Extended Data Fig 1b. b, HAE cells were infected with 2,000 E copies/cell of VIC. E copies were measured in apical washes of infected cultures. c, Calu-3 infection at 2,000 E copies/cell after 8h pre-treatment with IFNβ. Infection levels are shown normalized to untreated controls at 24 hpi. d, IFNβ and ISGs expression in HAE cells infected with 2,000 E copies/cell of IC19 or Alpha variant normalized to intracellular E copies for each sample. Shown are mean ± s.e.m. of one of three representative experiments performed in triplicate. For d, n = 6, two independent donors. Two Way ANOVA (a,c) or One Way ANOVA (d) with Wilcoxon matched-pairs signed rank test were used. Blue stars indicate comparison between Alpha and VIC (blue lines and symbols), grey stars indicate comparison between Alpha and IC19 (grey lines and symbols). * (p < 0.05), ** (p < 0.01), *** (p < 0.001), **** (p < 0.0001). ns: non-significant. E: viral envelope gene.
