Extended Data Fig. 9: Ex vivo brain Crz+ neurons respond to bath application of Dh31 peptide.
From: A nutrient-specific gut hormone arbitrates between courtship and feeding
Brain Crz+ neurons exhibited increased calcium activity in response to synthetic Dh31 peptide. The evoked response latency decreased with increasing concentration of Dh31, while the peak ∆F/F did not change. Ex vivo brain preparations of flies carrying Crz-GAL4 and UAS-GCaMP7s were imaged with two-photon microscopy. (a) Representative calcium responses (ΔF/F) of brain Crz+ neurons in response to different concentrations of Dh31 peptide. The time of Dh31 application is marked by an arrowhead below the calcium response (ΔF/F) trace. (b) Response latency (left) and peak ΔF/F (right) of brain Crz+ neurons in response to different concentrations of Dh31 peptide. Error bars, SEM. n = 7 samples. Significant differences (p < 0.05) are indicated by different letters, One-way ANOVA followed by Tukey’s test. Detailed statistical analysis is available in Supplementary Tables 7.
