Extended Data Fig. 1: STZ-induced hyperglycemia leads to reduced oocyte retrieval but does not affect the development potential of early embryos resulted.
From: Maternal inheritance of glucose intolerance via oocyte TET3 insufficiency

a, Gradually increased blood glucose levels after STZ treatment in female mice. Ctrl, HG: n = 16 and 26 mice, respectively. MII oocytes were collected for studies 30 days upon STZ injection (HG oocytes). b–c, Reduced GV (b) and MII (c) oocytes retrieval per mouse. Ctrl, HG: n = 6 mice per group for GV, and n = 14 independent experiments per group for MII, respectively. d, Granulosa cell dysfunction in HG mice evidenced by decreased serum estradiol (left) but increased free testosterone (right) concentration 48 h after PMSG priming. Ctrl, HG: n = 8 and 6 mice for estradiol; n = 8 and 8 mice for free testosterone, respectively. e, Smaller cumulus-oocyte complex (COC) in HG mice. Shown are representative images of COCs retrieved from control and hyperglycemic mice in 3 independent experiments with similar results. Scale bar, 500 μm. f, HG zygotes did not exhibit developmental delay at the timepoint 6 h post IVF when PN3–4 male pronuclei were collected. g–h, Maternal hyperglycemia did not significantly affect the 2-cell embryo formation (g) or blastocyst formation (h) in the IVF experiments. Ctrl, HG: n = 13 independent experiments per group. i, Representative images of oocytes and IVF-derived pre-implantation embryos in HG versus control group in 3 independent experiments with similar results. Scale bar, 100 μm. j, Normal litter size of offspring born from transferred 2-cell embryos derived from HG oocytes. Ctrl, HG: n = 20 and 23 deliveries, respectively. k, Normal appearance of HG E18.5 fetuses and placentas. Representative litters are shown. Scale bar, 2 cm. Data are presented as the mean ± s.e.m.; two-tailed P values were calculated by the unpaired (b, d, j) or paired (c, g, h) Student’s t-test. *P < 0.05, *P < 0.01  and ***P < 0.001. Statistical details are in Supplementary Table 5.