Extended Data Fig. 4: Normal insulin sensitivity and pancreas histomorphometry but defective insulin secretion in HG offspring.
From: Maternal inheritance of glucose intolerance via oocyte TET3 insufficiency
a–d, Normal insulin sensitivity in HG offspring fed either chow diet or HFD. Shown are ITT results of male (a) and female (b) with a chow diet, and male (c) and female (d) offspring after HFD induction at 16 weeks of age. a–d, Ctrl, HG: n = 16 and 12 mice for males; 13 and 12 mice for females. Ctrl-HFD and HG-HFD: n = 9 and 13 mice for males; 7 and 12 mice for females, respectively. e, The stimulation index of insulin release from isolated pancreatic islets of female offspring after HFD feeding at 24 weeks of age. Ctrl, HG: n = 9 per mouse. f–h, Insulin immunohistochemistry (IHC) (f), Hematoxylin and eosin (H&E) staining (g) and immunofluorescence (IF) (h) in paraffin-embedded pancreas sections from 16-week-old male offspring. Shown are representative images from 5 mice per group with similar results. No significant differences of islets in size and distribution (f), morphology (g) or α/β cell ratio (h) were detected between control and HG. Scale bar, 1 mm (f), 50 μm (g–h). i–k, Analysis of pancreatic morphology from 16-week-old male offspring by H&E staining. Ctrl, HG: n = 11 and 9 mice, respectively (with 2 sections per mouse). i, Average islet size. j, Islet density expressed as the average number of islets per 107 μm2 of pancreas area. k, Profiling of islet size distribution. l, Total pancreatic insulin content of 16-week-old male offspring. Ctrl, HG: n = 9 mice per group. a–e, i–l, Data are presented as the mean ± s.e.m.; two-tailed P values were calculated by one-way repeated-measurements ANOVA followed by the post hoc unpaired t-test (a–d) and the unpaired Student’s t-test (e, i–l). **P < 0.01. Statistical details are in Supplementary Table 5.
