Extended Data Fig. 2: PAX8 and HNF1B expression patterns in normal kidney and ccRCC.
From: The renal lineage factor PAX8 controls oncogenic signalling in kidney cancer
a. PAX8 and HNF1B mRNA expression as determined by RNA-seq in the GTex data set of normal tissues and the TCGA cancer data set. Median expression shown for each cancer and tissue type. b. PAX8 and HNF1B immunohistochemistry in ccRCC and normal kidney (representative images from TMA in panel e). Scale bar, 100μm. c. Western blot showing PAX8 and HNF1B expression in cell lines (N = 16 biological replicates of cells of renal origin, N = 3 biological replicates of cells of other origins; representative images, N = 2 technical replicates). d. PAX8 and HNF1B expression in ccRCCs when compared to normal kidney in the TCGA data set (Normal N = 72, T1 N = 252, T2 N = 66, T3-4 N = 189). Mean and SEM. Two-sided Kruskal-Wallis with Dunn’s multiple comparison test. e. Immunohistochemistry (IHC) for PAX8 and HNF1B expression in a tissue microarray of ccRCCs. N = 350 (PAX8), N = 361 (HNF1B). f. Unsupervised UMAP analysis of scRNA-seq data from fetal human kidney. Different cell types labelled in different colours. g. PAX8 and HNF1B expression across different cell types in the fetal human kidney. h. PAX8 and HNF1B expression in the cell types identified in scRNA-seq data from fetal human kidney.
