Extended Data Fig. 9: Diverse polarized receptors in C. elegans neurons are endocytosed in the AIS and require endocytosis for their polarity.
From: Endocytosis in the axon initial segment maintains neuronal polarity
(a) HPO-30-GFP localization in the PVD neuron. HPO-30-GFP is punctate in the AIS (inset). (b) HPO-30-GFP polarity index of animals described in a. (c) HPO-30-mCherry overlaps with CLIC-1-FLPon-GFP in the PVD neuron AIS. (d) HPO-30-mCherry overlaps with GFP-FLPon-RAB-7 in the PVD neuron AIS. (e) HPO-30-GFP in the axon of wild type or dynamin-1 temperature sensitive (TS) mutants grown at the permissive (20 °C) or restrictive temperatures (32 °C, 4 h). (f) HPO-30-GFP dendritic polarity of animals described in e. (g) CAM-1-GFP localization in the DA9 neuron. (h) CAM-1-GFP polarity index of animals described in g. (i) CAM-1-GFP colocalizes with mScarlet-FLPon-CLIC-1 in the DA9 neuron AIS (left). Outlined in black is a neighbouring cell body. (j) Clathrin-coated vesicle containing CAM-1-GFP in the AIS of the animal shown in i. Yellow box indicates cropped region. (k) CAM-1-GFP colocalizes with mRuby-RAB-7 in the DA9 neuron AIS. Outlined in black is a neighbouring cell body. (l) RAB-7 labelled vesicle containing CAM-1-GFP in the AIS of animal shown in k. Yellow box indicates cropped region. (m) CAM-1-GFP mislocalizes to the axon in apa-2 loss of function mutant animals. (n) CAM-1-GFP polarity index of animals described in k. Wild-type data are from Extended Data Fig. 9h. All data are shown as mean ± s.e.m. N-values are indicated on bar graphs and represent the number of individual animals. P values in n were calculated using a two-tailed unpaired t test. P values in f were calculated using a two-way ANOVA with Šidák multiple comparison test. Scale bars, 10 µm (a, c top, d top, g), 5 µm (i, k), 2 µm (e), 1 µm (a inset, c bottom, d bottom, j, l, m).
