Fig. 3: Endocytosis maintains dendritic receptor compartmentalization and is critical for neuronal function.
From: Endocytosis in the axon initial segment maintains neuronal polarity
a, Localization of endogenously labelled DMA-1–FLPon–GFP in the axon of wild-type or dynamin-1 temperature-sensitive C. elegans animals. Scale bar, 2 µm. b, DMA-1–FLPon–GFP polarity index of C. elegans animals described in a. c, Confocal images of DIV26 human neurons treated with vehicle control (DMSO) or the endocytosis inhibitor Dyngo 4a for 18 h prior to fixation and staining for the indicated endogenous proteins. Arrows indicate axonal regions. Scale bars, 20 µm. d, TfR fluorescence in the axon of neurons described in c. Scale bars, 5 µm. e, Average TfR fluorescence intensity in the dendrite and axon of neurons described in c. f, Localization of endogenous DMA-1–FLPon–GFP in axons of wild-type and endocytic mutant C. elegans animals. Scale bar, 2 µm. g, DMA-1–FLPon–GFP polarity index of C. elegans animals described in f. h, A PVD axon labelled with a myristoylated GFP membrane marker in C. elegans animals of the indicated genotype. Scale bars, 5 µm. i, Number of axonal branches in the 50 µm distal to the AIS in C. elegans animals of the indicated genotype. j, Escape behaviour in C. elegans animals in response to a harsh touch stimulus. Wild-type and degt-1 data are from Fig. 1p. Data are mean ± s.e.m. n represents the number of individual animals or cells for each condition. b,e, Two-way ANOVA with Šidák's multiple comparison test. g, Brown–Forsythe and Welch one-way ANOVA with Dunnett's test. j, One-way ANOVA with Dunnett’s test.
