Extended Data Fig. 4: (related to Fig 3): ILC2s regulate eosinophils in tissue.
From: Non-redundant functions of group 2 innate lymphoid cells
Analysis of eosinophils across organs in the steady state. a, Eosinophils were gated from live CD45+ Lin− (CD3, CD5, CD19, Ly6G) cells using SiglecF and CCR3, across organs of Nmur1iCre-eGFP Id2fl/fl mice and littermate controls. b, Relative ILC2 and eosinophil numbers in bone marrow chimera of Nmur1iCre-eGFP Id2fl/fl (KO) and Id2fl/fl (WT) recipients transplanted with bone marrow of Nmur1iCre-eGFP Id2fl/fl (KO) or Id2fl/fl (WT) donors. c, Quantification of eosinophils in IL5−/− mice and respective IL5+/− littermate controls. d, Flow cytometry plots of granulocyte/macrophage progenitor (GMP) gated from live CD45+ Lin− (CD3, CD5, CD19, Gr1, B220) SCA-1− c-Kithi cells as CD34+ CD16/CD32hi and relative quantification in bone marrow of Nmur1iCre-eGFP Id2fl/fl mice and Id2fl/fl littermate controls. e, Quantification of relative Eosinophil precursors (EoP) and GMPs in the bone marrow of IL-5−/− compared with WT controls. f, Flow-cytometric quantification of relative numbers of bone marrow-derived eosinophils gated from live CD45+ Lin− (CD3, CD5, CD19) CD11b+ cells as SSChi and SiglecF+. BM cells were cultured with either SCF and FLT3L, IL-5, ILC2P or supernatant from ILC2s for 10 days. g,h Mean fluorescence intensity of CCR3 on eosinophils in Nmur1Cre-T2A-GFP Id2flox/flox (g) and Il5−/− (h) mice and respective littermate controls. b-e, g-h One symbol represents data from one mouse (b-e, g-h) or one well (f), data are mean ± s.d., c—e, g-h Student‘s t-test. b, f One-way ANOVA with Tukey’s multiple comparisons, NS not significant, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
