Extended Data Fig. 12: Stimulation of peripheral endocannabinoid receptors drives exercise performance.
From: A microbiome-dependent gut–brain pathway regulates motivation for exercise
a-d, Averaged hourly distance (a, c) and quantifications (b, d) of voluntary wheel activity of Abx-treated mice and controls, with or without treatment with the CB1 inhibitor O-2050 (a, b) or the CB1 agonist CP55,940 (c, d). Insets show representative recording traces. e-h, Kaplan-Meier plots (e, g) and quantifications (f, h) of distance (e, f) and time (g, h) on treadmills by Abx-treated mice and controls, with or without treatment with the peripheral CB1 inhibitor AM6545. i-m, Fos expression in DRGs (i), Kaplan-Meier plots (j, l) and quantifications (k, m) of distance (j, k) and time (l, m) on treadmills by GF mice and GF mice mono-colonized with E. coliereA−T, with and without AM6545 treatment. n, o, Expression of Maoa (n) and dopamine levels in the striatum (o) of AM6545-treated mice after treadmill exercise. p, Schematic of pathway model linking the intestinal microbiome to exercise performance. q, r, Latency to paw withdrawal on a hot plate by Abx-treated mice and controls before and after exercise (q) and after exercise, with and without AM6545 treatment (r). Error bars indicate means ± SEM. * p < 0.05, *** p < 0.001, **** p < 0.0001. Exact n and p-values are presented in Supplementary Table 2.
