Fig. 2: The efficacy of 3-IAA and FIRINOX is licensed by MPO.
From: Microbiota-derived 3-IAA influences chemotherapy efficacy in pancreatic cancer
a, SPF mice were orthotopically injected with KPC cells and treated with FIRINOX with or without 3-IAA, and tumours were analysed at day three after FIRINOX treatment (n = 8 each). Immune subsets of orthotopic tumours or respective spleens were determined by flow cytometry. CD8+ T cells (CD3+CD8+); CD4+ T cells (CD3+CD4); macrophages (CD11b+F4/80+); neutrophils (CD11b+Ly6G+) are shown as relative to total living immune cells (CD45+). b, Tumour weight and counts of immune subsets of orthotopic KPC tumours or respective spleens of irradiated and wild-type (WT; n = 4) or Mpo–/– (n = 5 or 6) bone-marrow (BM)-reconstituted mice treated and analysed as in a. c, Irradiated and wild-type, Mpo–/– or Ahr–/– bone-marrow-reconstituted mice received KPC cells orthotopically and were treated with FIRINOX or FIRINOX + 3-IAA for five days (n = 5 each). All mice received a four-day dietary intervention with a high-tryptophan diet. Tumour weight was assessed at day seven after FIRINOX treatment. Each symbol represents one mouse. Two independent experiments were pooled (a) or one out of two independent experiments (b,c) is shown. Error bars indicate s.e.m. Significant P values are indicated and were determined by two-tailed Mann–Whitney test (a) or two-tailed t-test (b,c).
