Extended Data Fig. 5: Analysis of high-speed timelapse SEPT6-GFP data in MV3.
From: Blebs promote cell survival by assembling oncogenic signalling hubs
(A) Autocorrelation curves for the local mean curvature and SEPT6-GFP data presented in Fig. 2l; the fluorescence signal was normalized to account for photobleaching as described in Methods. (B) Temporal cross-correlation functions as shown in Fig. 2l, here with extended range of time lags. (C) Mean rates of absolute change in SEPT6-GFP intensity for positive and negative intracellular mean curvature contigs, expressed as a function of the dynamicity of the contigs they occur within. Dynamicity is defined as the total number of timepoints showing intensity increases and decreases that occur within a contig, divided by the total number of timepoints within that contig. Derived from the same dataset used in Fig. 2l. Vertical dashed line represents the threshold for low and high dynamic groups as shown in Figs. 2l and S7B.
