Extended Data Fig. 7: Changes in septin expression upon MAPKi treatment of A375 cells.
From: Blebs promote cell survival by assembling oncogenic signalling hubs
Top, Western blots of SEPT2, SEPT6, SEPT7, and SEPT9 abundance (the 4 septins with highest expression in A375 cells, as measured by mass spectrometry, see Extended Data Table 2) upon combined treatment with Dabrafenib and Trametinib at doses indicated. Three independent experiments were performed, with data from all replicates shown alongside vinculin loading controls. Bottom, densitometry analysis of Western blots showing fold change in septin expression as dose response curves. Dashed lines indicate individual experiments (color-coded according to the color bars indicating Western blot replicates); solid red lines indicate means of all replicates.
